Transport properties of membrane vesicles from Acholeplasma laidlawii. I. Isolation and general characteristics
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
127739
DOI
10.1007/bf02891706
Knihovny.cz E-zdroje
- MeSH
- Acholeplasma laidlawii enzymologie metabolismus MeSH
- adenosintrifosfatasy metabolismus MeSH
- buněčná membrána enzymologie metabolismus MeSH
- frakcionace buněk MeSH
- glukokinasa metabolismus MeSH
- glukosa metabolismus MeSH
- metabolismus sacharidů MeSH
- NADH, NADPH oxidoreduktasy metabolismus MeSH
- spotřeba kyslíku MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- adenosintrifosfatasy MeSH
- glukokinasa MeSH
- glukosa MeSH
- NADH, NADPH oxidoreduktasy MeSH
A new procedure for the isolation of membrane vesicles from Acholeplasma laidlawii cells is described. The membrane vesicles are completely free from contaminations of whole cells and cell debris and represent a homogeneous fraction as shown by electron microscopy, Ficoll density-gradient centrifugation, and titration on agar plate. Absence of cytoplasmic contaminations was confirmed by double-labelling of membranes with 3H-oleic acid and 14C-uridine, as well as by distribution of specific marker enzymes of membranes and cytoplasm. On the basis of light-scattering and electron microscopy, the vesicular nature of these membranes was established. The vesicles had the same orientation as intact cells (absence on membrane vesicles of ATPase and NADH dehydrogenase activities, localized in the inner surface of membrane). The respiratory activity of the membrane vesicles was low and was not stimulated by exogenous substrates, the respiratory chain of the vesicles being reduced and terminated by flavoproteins. The ability of membrane vesicles to take up carbohydrates was shown.
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