Transport properties of membrane vesicles from Acholeplasma laidlawii. II. Kinetic characteristics and specificity of glucose transport system
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
286
DOI
10.1007/bf02891707
Knihovny.cz E-zdroje
- MeSH
- Acholeplasma laidlawii metabolismus MeSH
- aktivní transport MeSH
- buněčná membrána metabolismus MeSH
- deoxyglukosa metabolismus MeSH
- glukosa metabolismus MeSH
- kinetika MeSH
- koncentrace vodíkových iontů MeSH
- maltosa metabolismus MeSH
- methylglukosidy metabolismus MeSH
- subcelulární frakce MeSH
- teplota MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- deoxyglukosa MeSH
- glukosa MeSH
- maltosa MeSH
- methylglukosidy MeSH
The glucose transport system of membrane vesicles isolated from Acholeplasma laidlawii is saturable, with a Km of 21.2 mum and V of 0.68 nmol min-1 (mg protein)-1. The process is pH-dependent and a break occurs in the Arrhenius plot at 15 degrees C. Exogenous substrates did not stimulate glucose transport probably due to their inability to penetrate into membrane vesicles. 3-O-Methylglucose and 6-deoxyglucose competitively inhibited glucose transport. Maltose inhibited transport of glucose noncompetitively. These sugars also elicited glucose efflux from preloaded membrane vesicles.
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