Production of alkaloids and differentiation in a submerged culture of Claviceps purpurea (Fr.) Tul
Language English Country United States Media print
Document type Journal Article
PubMed
240760
DOI
10.1007/bf02876766
Knihovny.cz E-resources
- MeSH
- Acetyl-CoA Carboxylase metabolism MeSH
- Claviceps enzymology growth & development metabolism MeSH
- Fermentation MeSH
- Ricinoleic Acids biosynthesis MeSH
- Fatty Acids biosynthesis MeSH
- Ergot Alkaloids biosynthesis MeSH
- Spores, Fungal enzymology growth & development metabolism MeSH
- Tryptophan biosynthesis MeSH
- Tryptophan Synthase metabolism MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Acetyl-CoA Carboxylase MeSH
- Ricinoleic Acids MeSH
- Fatty Acids MeSH
- Ergot Alkaloids MeSH
- Tryptophan MeSH
- Tryptophan Synthase MeSH
The submerged culture of the Claviceps purpurea strain studied was polymorphous. In the process of alkaloid synthesis, cytodifferentiation preceded biochemical differentiation. The onset of the alkaloid phase was characterized by: predominance of chlamydospores in the culture, the presence of vegetative cells with reduced or arrested proliferation, maximum acetylCoA carboxylase activity, the maximum amount of total fatty acids, an over-average cell pool tryptophan level and minimum tryptophan synthetase activity. Intracellular ricinoleic acid was an indicator of differentiation of the culture towards alkaloid formation and also of alkaloid synthesis. The cytodifferentiation period in the initial phases of fermentation, when the cell has several alternative possibilities of development, is regarded as the most sensitive sector of the regulatory mechanisms of alkaloid formation.
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