An auxotrophic strain of Escherichia coli with the recB recC sbcB genotype was transformed by chromosomal DNA of the prototrophic strain and by plasmid DNA carrying genes for antibiotic resistance (R1 drd19). The donor plasmid DNA obtained by cell lysis in the presence of Triton X-100 and subsequent centrifugation in a caesium chloride-ethidium bromide gradient was shown to have a circular molecule and to retain its completeness after penetration into the recipient. Experiments with mixtures or plasmid and chromosomal DNA indicate a competition between these two DNA types during the transformation reaction in the given system.

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Convenience of plasmid R6K higher-copy-number deletion derivative for cloning of larger DNA fragments

An in vivo cointegrate of two plasmids from incompatibility group X

New replication mutant pNH602 and its relationship to plasmid pAS3, another deletion derivative of plasmid R6K

Isolation and characterization of a higher-copy-number mutant of plasmid R6K

Visualization of membrane-associated R-plasmid DNA in fraction of Escherichia coli minicell lyzate

Application of R-plasmid DNA's from Escherichia coli minicells in genetic transformation