A stable deletion derivative pNH602 was obtained when the recently described higher-copy-number point mutant pNH601 of plasmid R6K was introduced to a minicells-producing strain of Escherichia coli. The size of plasmid pNH602 is 18.8 Mg/mol as determined by electron microscopy. The 7.2 Mg/mol fragment of R6K genome missing in pNH602 carries the Smr-determinant and the region finO and, according to the results of restriction analysis, it includes one EcoRI site. With its radioisotopically determined 33 copies of pNH602 per E. coli K-12 chromosome (npc), representing a 23% increase of the point mutant pNH601 and 150% enhancement of R6K npc, plasmid pNH602 differs from another closely related R6K deletion derivative pAS3 of the same size which exhibits only 20 npc. Both pNH602 and pAS3 plasmids are conjugative.

Zobrazit více v PubMed

Mol Gen Genet. 1975 Sep 15;140(1):39-50 PubMed

Plasmid. 1978 Sep;1(4):584-8 PubMed

Folia Microbiol (Praha). 1982;27(2):138-41 PubMed

J Gen Microbiol. 1982 Mar;128(3):529-37 PubMed

Nucleic Acids Res. 1977;4(5):1465-82 PubMed

Plasmid. 1981 Jan;5(1):2-9 PubMed

Folia Microbiol (Praha). 1983;28(5):345-52 PubMed

J Bacteriol. 1979 Feb;137(2):977-89 PubMed

Microbiol Rev. 1983 Jun;47(2):180-230 PubMed

Virology. 1980 Nov;107(1):89-99 PubMed

J Bacteriol. 1975 Jul;123(1):28-35 PubMed

Genetika. 1981;17(9):1555-65 PubMed

Genetika. 1978 Nov;14(11):1863-75 PubMed

J Gen Microbiol. 1972 Oct;72(3):543-52 PubMed

J Mol Biol. 1968 Feb 14;31(3):519-40 PubMed

Genetika. 1982;18(8):1263-70 PubMed

J Bacteriol. 1973 May;114(2):577-91 PubMed

Mol Gen Genet. 1982;187(2):187-94 PubMed

Plasmid. 1980 Sep;4(2):170-4 PubMed

Folia Microbiol (Praha). 1977;22(5):353-9 PubMed

Convenience of plasmid R6K higher-copy-number deletion derivative for cloning of larger DNA fragments

Mini-Mu transposition of bacterial genes on the transmissible plasmid

An in vivo cointegrate of two plasmids from incompatibility group X