An economic method for a rapid estimation of the number of copies of plasmid R6K delta 1 in E. coli cells using lysis of the cells directly on the agarose gel and electrophoretic separation of radioactively labelled plasmid and chromosomal DNA's is described. The method, particularly useful for screening purposes, permits detection of both the CCC and OC forms of plasmids of molar mass 2-150 Mg/mol and it can be applied to other bacterial systems.

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General and molecular microbiology and microbial genetics in the IM CAS

Effect of temperature on alpha-amylase formation and DNA replication in Bacillus subtilis

Convenience of plasmid R6K higher-copy-number deletion derivative for cloning of larger DNA fragments

An in vivo cointegrate of two plasmids from incompatibility group X

New replication mutant pNH602 and its relationship to plasmid pAS3, another deletion derivative of plasmid R6K

Isolation and characterization of mutants of the RP4 plasmid coding for increased resistance to ampicillin

Isolation and characterization of a higher-copy-number mutant of plasmid R6K