The study of the role of mobile elements and mobilization of resistance genes is crucial for understanding the epidemiology of antibiotic resistance. This review summarizes recent data on the insertion sequences, transposons, integrons and plasmids that are involved in the mobilization of bacterial antibiotic resistance genes.
- Klíčová slova
- inserční sekvence, transpozon, integron, plazmid,
- MeSH
- antibiotická rezistence MeSH
- bakteriální léková rezistence genetika MeSH
- chromozomální inverze MeSH
- integrony genetika MeSH
- inzerční mutageneze MeSH
- R-plasmidy genetika MeSH
- transpozibilní elementy DNA genetika MeSH
- Publikační typ
- přehledy MeSH
- MeSH
- Escherichia coli genetika izolace a purifikace MeSH
- konjugace genetická MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- plazmidy genetika izolace a purifikace ultrastruktura MeSH
- R-plasmidy genetika izolace a purifikace MeSH
- restrikční mapování MeSH
- transformace genetická MeSH
- Check Tag
- lidé MeSH
The plasmid vector pEM100 (13.5 kb) constructed from pGV1106, a miniderivative of the broad-host-range IncW pSa plasmid, and the pAM330 plasmid of Brevibacterium lactofermentum is not stably maintained in Escherichia coli host cells under nonselective growth conditions. By insertion of a 0.9 kb DNA fragment containing the parB locus (responsible for the maintenance of plasmid R1 in E. coli cells) to plasmid pEM100, plasmid pEM110 was prepared which is maintained in a population of E. coli cells growing without a selection pressure very stably.
- MeSH
- antibakteriální látky škodlivé účinky terapeutické užití MeSH
- Bacteria genetika patogenita účinky léků MeSH
- bakteriální léková rezistence * genetika účinky léků MeSH
- dějiny 20. století MeSH
- infekce spojené se zdravotní péčí * mikrobiologie prevence a kontrola MeSH
- Klebsiella pneumoniae genetika patogenita MeSH
- lidé MeSH
- mikrobiologické techniky klasifikace metody MeSH
- nemocniční oddělení MeSH
- plazmidy analýza genetika klasifikace MeSH
- R-plasmidy analýza genetika MeSH
- Check Tag
- dějiny 20. století MeSH
- lidé MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- antibakteriální látky farmakologie MeSH
- antibiotická rezistence MeSH
- Enterobacteriaceae účinky léků genetika izolace a purifikace MeSH
- lidé MeSH
- městské obyvatelstvo MeSH
- mikrobiologie vody * MeSH
- odpad tekutý - odstraňování * MeSH
- odpadní vody * MeSH
- R-plasmidy * MeSH
- Salmonella účinky léků genetika izolace a purifikace MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Československo MeSH
Three strains belonging to gramnegative non-fermenting rods, i.e. a Pseudomonas maltophilia strain and two strains of Acinetobacter, were tested, as representatives of different types of nosocomial strains, for transferability of their multiple drug resistance. As all of them posed difficulties in demonstrating the transferability of their resistance by conventional methods, a three-step procedure was developed that includes a transfer to rifampicin-resistant P. aeruginosa recipients, then to susceptible P. aeruginosa intermediate strains, and, finally, from these strains to rifampicin-resistant Enterobacteriaceae. In three strains studied three genetically different types of R plasmids have been demonstrated. P. maltophilia transferred Amikacin resistance, as well as resistance to other antibiotics, to P. aeruginosa and then to Enterobacteria. In contrast, an Amikacin-resistant Acinetobacter with quite identical multiple drug resistance spectrum transferred its resistance to P. aeruginosa only, but not to Enterobacteria. Finally, another Acinetobacter strain, resistant to Gentamicin but susceptible to Amikacin transferred this resistance directly to Enterobacteria (and, separately, to P. aeruginosa, too). All three strains transferred Cefamandole resistance together with other resistances. Non-fermenting rods, thus, might be a source of transmissible resistance to reserve antibiotics as Amikacin, and advanced-type Cephalosporins.
- MeSH
- Acinetobacter účinky léků genetika MeSH
- amikacin farmakologie MeSH
- cefamandol farmakologie MeSH
- Enterobacteriaceae genetika MeSH
- Escherichia coli genetika MeSH
- kanamycin analogy a deriváty MeSH
- Pseudomonas aeruginosa genetika MeSH
- Pseudomonas účinky léků genetika MeSH
- R-plasmidy * MeSH
- Salmonella typhimurium genetika MeSH
- Publikační typ
- časopisecké články MeSH
