Isolation and characterization of mutants of the RP4 plasmid coding for increased resistance to ampicillin
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
2997006
DOI
10.1007/bf02928748
Knihovny.cz E-zdroje
- MeSH
- ampicilin farmakologie MeSH
- druhová specificita MeSH
- Escherichia coli účinky léků genetika MeSH
- ethylmethansulfonát farmakologie MeSH
- mutace * MeSH
- R-plasmidy účinky léků MeSH
- replikace DNA účinky léků MeSH
- restrikční enzymy MeSH
- rezistence na penicilin MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- ampicilin MeSH
- ethylmethansulfonát MeSH
- restrikční enzymy MeSH
E. coli strain J53(RP4) was mutagenized with ethyl methanesulfonate and N-methyl-N'-nitro-N-nitrosoguanidine. Clones showing a two-to threefold increase in resistance to ampicillin were produced. This increase was not due to an increased number of RP4 copies per chromosome. The level of penicillinase activity was twice higher in comparison with the parental strain. No detectable changes were found in the region coding for the resistance to ampicillin on the plasmid by restriction analysis.
Zobrazit více v PubMed
J Bacteriol. 1951 Sep;62(3):293-300 PubMed
Plasmid. 1983 Nov;10(3):303-7 PubMed
Biochem J. 1971 Jul;123(4):493-500 PubMed
Folia Microbiol (Praha). 1982;27(2):138-41 PubMed
Plasmid. 1981 Jan;5(1):10-9 PubMed
Nucleic Acids Res. 1978 Jul;5(7):2373-80 PubMed
Biochem J. 1962 May;83:236-40 PubMed
J Mol Biol. 1979 Sep 25;133(3):295-318 PubMed
Genet Res. 1968 Oct;12(2):157-68 PubMed
Plasmid. 1983 Sep;10(2):184-95 PubMed
J Bacteriol. 1976 Feb;125(2):635-42 PubMed
J Gen Microbiol. 1971 May;66(2):205-14 PubMed
