Interaction of granaticin B with the transcription system of Bacillus subtilis
Language English Country United States Media print
Document type Journal Article
PubMed
411719
DOI
10.1007/bf02877667
Knihovny.cz E-resources
- MeSH
- Adenosine Triphosphate MeSH
- Bacillus subtilis growth & development metabolism MeSH
- RNA, Bacterial biosynthesis MeSH
- Bacteriophages MeSH
- Benzofurans pharmacology MeSH
- DNA-Directed RNA Polymerases metabolism MeSH
- DNA MeSH
- Transcription, Genetic drug effects MeSH
- Templates, Genetic MeSH
- Magnesium MeSH
- Spectrophotometry MeSH
- Terpenes pharmacology MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Adenosine Triphosphate MeSH
- RNA, Bacterial MeSH
- Benzofurans MeSH
- DNA-Directed RNA Polymerases MeSH
- DNA MeSH
- Magnesium MeSH
- Terpenes MeSH
The interaction of granaticin B, a quinone antibiotic produced by Streptomyces granaticolor, with some biologically important bivalent metal ions, DNA and ATP was demonstrated spectrophotometrically. The activity of isolated RNA polymerase was higher when the DNA of phage SP 50 served as template than with DNA isolated from Bacillus subtilis. Granaticin B inhibited in vitro RNA synthesis, similarly to certain other antibiotics (the inhibition was three times lower than that caused by actinomycin D or streptolydigin and slightly higher than that by epsilon-pyrromycinone). The inhibitory effect was higher when the Mg2+ concentration in the reaction mixture was decreased. The inhibition was then proportional to the concentration of the DNA template. DNA-dependent RNA synthesis is thus inhibited in vitro by granaticin B but this does not appear to be the only site of action of this antibiotic in vivo.
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