A gene (hur) from Streptomyces aureofaciens, conferring resistance to hydroxyurea, is related to genes encoding streptomycin phosphotransferase
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články
PubMed
1316866
DOI
10.1016/0378-1119(92)90719-6
PII: 0378-1119(92)90719-6
Knihovny.cz E-zdroje
- MeSH
- antibiotická rezistence genetika MeSH
- bakteriální geny MeSH
- Escherichia coli genetika MeSH
- fosfotransferasy s alkoholovou skupinou jako akceptorem * MeSH
- fosfotransferasy chemie genetika MeSH
- hydroxymočovina farmakologie MeSH
- molekulární sekvence - údaje MeSH
- rekombinantní DNA genetika MeSH
- restrikční mapování MeSH
- ribonukleotidreduktasy genetika MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- sekvenční homologie nukleových kyselin MeSH
- Streptomyces aureofaciens enzymologie genetika MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fosfotransferasy s alkoholovou skupinou jako akceptorem * MeSH
- fosfotransferasy MeSH
- hydroxymočovina MeSH
- rekombinantní DNA MeSH
- ribonukleotidreduktasy MeSH
- streptomycin 3''-kinase MeSH Prohlížeč
A novel gene (hur) conferring resistance to hydroxyurea (HU) in Escherichia coli has been identified in a Streptomyces aureofaciens genomic library. The expression of hur in E. coli was under the control of the external plasmid tet promoter. Sequence analysis of a minimal fragment revealed an open reading frame (ORF) encoding a protein of 340 amino acids with an M(r) of 36,049 and an average hydropathy index of 1.13. The predicted protein product was similar to streptomycin phosphotransferases from Streptomyces glaucescens and Streptomyces griseus (52.4% and 50.8% identity, respectively), but it did not confer resistance to streptomycin or to any of the other aminoglycoside antibiotics tested. It is inferred that hur encodes a phosphotransferase that inactivates HU by phosphorylation of the hydroxy group in the hydroxylamine moiety.
Citace poskytuje Crossref.org
GENBANK
M76682, M79307, M79308, M79309, M79310, M81739, M83744, X58743, X60732, X60733
