A gene (hur) from Streptomyces aureofaciens, conferring resistance to hydroxyurea, is related to genes encoding streptomycin phosphotransferase
Language English Country Netherlands Media print
Document type Journal Article
PubMed
1316866
DOI
10.1016/0378-1119(92)90719-6
PII: 0378-1119(92)90719-6
Knihovny.cz E-resources
- MeSH
- Drug Resistance, Microbial genetics MeSH
- Genes, Bacterial MeSH
- Escherichia coli genetics MeSH
- Phosphotransferases (Alcohol Group Acceptor) * MeSH
- Phosphotransferases chemistry genetics MeSH
- Hydroxyurea pharmacology MeSH
- Molecular Sequence Data MeSH
- DNA, Recombinant genetics MeSH
- Restriction Mapping MeSH
- Ribonucleotide Reductases genetics MeSH
- Amino Acid Sequence MeSH
- Base Sequence MeSH
- Sequence Homology, Nucleic Acid MeSH
- Streptomyces aureofaciens enzymology genetics MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Phosphotransferases (Alcohol Group Acceptor) * MeSH
- Phosphotransferases MeSH
- Hydroxyurea MeSH
- DNA, Recombinant MeSH
- Ribonucleotide Reductases MeSH
- streptomycin 3''-kinase MeSH Browser
A novel gene (hur) conferring resistance to hydroxyurea (HU) in Escherichia coli has been identified in a Streptomyces aureofaciens genomic library. The expression of hur in E. coli was under the control of the external plasmid tet promoter. Sequence analysis of a minimal fragment revealed an open reading frame (ORF) encoding a protein of 340 amino acids with an M(r) of 36,049 and an average hydropathy index of 1.13. The predicted protein product was similar to streptomycin phosphotransferases from Streptomyces glaucescens and Streptomyces griseus (52.4% and 50.8% identity, respectively), but it did not confer resistance to streptomycin or to any of the other aminoglycoside antibiotics tested. It is inferred that hur encodes a phosphotransferase that inactivates HU by phosphorylation of the hydroxy group in the hydroxylamine moiety.
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GENBANK
M76682, M79307, M79308, M79309, M79310, M81739, M83744, X58743, X60732, X60733
