Relationship between cardiolipin content and cytochrome c oxidase activity of cytochrome aa3 in Paracoccus denitrificans
Language English Country Australia Media print
Document type Journal Article
PubMed
1332723
Knihovny.cz E-resources
- MeSH
- Aerobiosis MeSH
- Anaerobiosis MeSH
- Heme metabolism MeSH
- Cardiolipins metabolism MeSH
- Oxygen metabolism MeSH
- Paracoccus denitrificans growth & development metabolism MeSH
- Electron Transport Complex IV metabolism MeSH
- Substrate Specificity MeSH
- Tetramethylphenylenediamine MeSH
- Electron Transport MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Heme MeSH
- Cardiolipins MeSH
- Oxygen MeSH
- Electron Transport Complex IV MeSH
- Tetramethylphenylenediamine MeSH
Using cultivation at different oxygen tensions, the molar ratio of cardiolipin to haem a in cells of Paracoccus denitrificans was varied systematically from 30 to 300. The molecular activity of cytochrome aa3 (with N, N, N', N'-tetramethyl-p-phenylenediamine as substrate) remained unchanged in this interval, this ruling out any regulatory effect of physiological cardiolipin levels on the terminal oxidase. Titration of anaerobically grown cells with cyanide indicated the presence of cytochrome aa3 which accounted for about 1/4 of the total electron flow from TMPD to oxygen.
