Differential sensitivity of CG and CCG DNA sequences to ethionine-induced hypomethylation of the Nicotiana tabacum genome
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu srovnávací studie, časopisecké články
PubMed
1372869
DOI
10.1016/0014-5793(92)80860-j
PII: 0014-5793(92)80860-J
Knihovny.cz E-zdroje
- MeSH
- azacytidin farmakologie MeSH
- cytosinnukleotidy chemie MeSH
- DNA chemie účinky léků MeSH
- ethionin farmakologie MeSH
- genom MeSH
- guaninnukleotidy chemie MeSH
- jedovaté rostliny * MeSH
- metylace účinky léků MeSH
- repetitivní sekvence nukleových kyselin účinky léků MeSH
- tabák účinky léků genetika MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- azacytidin MeSH
- cytosinnukleotidy MeSH
- DNA MeSH
- ethionin MeSH
- guaninnukleotidy MeSH
Plant DNA is distinguished from the DNA of all other organisms by its high content of 5-methylcytosine (5mC). 5mC levels may amount to 30% of total cytosines, distributed between the sequences CG and CXG. The results presented here show that the methylation status of CXG sequences could be influenced by culturing tobacco tissues on subtoxic concentrations of ethionine. The hypomethylating effect of ethionine, evaluated as the capability of MspI or HpaII to cleave the DNA, proved to be rather specific for CCG and differed from that of 5-azacytidine which did not discriminate between CG and CXG sequences.
Citace poskytuje Crossref.org
Epigenetic control of sexual phenotype in a dioecious plant, Melandrium album
Meiotic transmission of a hypomethylated repetitive DNA family in tobacco
