Differential sensitivity of CG and CCG DNA sequences to ethionine-induced hypomethylation of the Nicotiana tabacum genome
Language English Country Great Britain, England Media print
Document type Comparative Study, Journal Article
PubMed
1372869
DOI
10.1016/0014-5793(92)80860-j
PII: 0014-5793(92)80860-J
Knihovny.cz E-resources
- MeSH
- Azacitidine pharmacology MeSH
- Cytosine Nucleotides chemistry MeSH
- DNA chemistry drug effects MeSH
- Ethionine pharmacology MeSH
- Genome MeSH
- Guanine Nucleotides chemistry MeSH
- Plants, Toxic * MeSH
- Methylation drug effects MeSH
- Repetitive Sequences, Nucleic Acid drug effects MeSH
- Nicotiana drug effects genetics MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- Azacitidine MeSH
- Cytosine Nucleotides MeSH
- DNA MeSH
- Ethionine MeSH
- Guanine Nucleotides MeSH
Plant DNA is distinguished from the DNA of all other organisms by its high content of 5-methylcytosine (5mC). 5mC levels may amount to 30% of total cytosines, distributed between the sequences CG and CXG. The results presented here show that the methylation status of CXG sequences could be influenced by culturing tobacco tissues on subtoxic concentrations of ethionine. The hypomethylating effect of ethionine, evaluated as the capability of MspI or HpaII to cleave the DNA, proved to be rather specific for CCG and differed from that of 5-azacytidine which did not discriminate between CG and CXG sequences.
References provided by Crossref.org
Epigenetic control of sexual phenotype in a dioecious plant, Melandrium album
Meiotic transmission of a hypomethylated repetitive DNA family in tobacco
