A recA-ada hybrid gene inducible by DNA damage
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
1374156
DOI
10.1016/0165-7992(92)90071-o
PII: 0165-7992(92)90071-O
Knihovny.cz E-resources
- MeSH
- Escherichia coli genetics MeSH
- Genetic Vectors * MeSH
- Cloning, Molecular * MeSH
- Methyltransferases genetics MeSH
- O(6)-Methylguanine-DNA Methyltransferase MeSH
- Plasmids MeSH
- DNA Damage * MeSH
- Rec A Recombinases genetics MeSH
- Regulatory Sequences, Nucleic Acid MeSH
- SOS Response, Genetics genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Methyltransferases MeSH
- O(6)-Methylguanine-DNA Methyltransferase MeSH
- Rec A Recombinases MeSH
A damage-inducible expression vector was constructed in which the original recA structural gene was replaced by the protein-coding region of the ada gene. The O6-alkylguanine-DNA alkyltransferase encoded by the ada gene can be measured by a rapid and highly sensitive assay. The introduction of this construct into an appropriate host cell provides an effective bacterial assay for genotoxins.
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