A new Type II restriction endonuclease ApaCI purified from Acetobacter pasteurianus is an isoschizomer of BamHI that cleaves at the nucleotide sequence 5'-G/GATCC-3' of double-stranded DNA. The single restriction activity present in this strain permits rapidly purified 30,000 units of cleavage activity from 10 g of freshly harvested cells. The resulting ApaCI preparation is free of contaminant nuclease activities that might interfere with in vitro manipulation of DNA.

Department of Biochemistry Faculty of Science Comenius University Bratislava Czechoslovakia

See more in PubMed

Methods Enzymol. 1983;101:20-78 PubMed

Nucleic Acids Res. 1988 Mar 25;16(6):2718 PubMed

J Mol Biol. 1975 Jan 25;91(3):315-28 PubMed

Nucleic Acids Res. 1983 Jul 11;11(13):4409-15 PubMed

Nucleic Acids Res. 1982 Oct 11;10(19):5747-52 PubMed

Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PubMed

Gene. 1977;2(2):95-113 PubMed

Gene. 1990 Aug 16;92(1-2):1-248 PubMed

Nucleic Acids Res. 1990 Apr 25;18 Suppl:2331-65 PubMed

Gene. 1985;33(1):103-19 PubMed

Connection between foreign DNA replication and induced expression of the restriction-modification system in Streptomyces aureofaciens