32P-postlabeling analysis of adducts formed from 1-phenylazo-2-hydroxynaphthalene (Sudan I, Solvent Yellow 14) with DNA and homopolydeoxyribonucleotides
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články
PubMed
1638690
DOI
10.1093/carcin/13.7.1221
Knihovny.cz E-zdroje
- MeSH
- autoradiografie MeSH
- chromatografie na tenké vrstvě MeSH
- DNA chemie MeSH
- endonukleasy specifické pro jednořetězcové nukleové kyseliny MeSH
- karcinogeny chemie MeSH
- naftoly chemie MeSH
- polydeoxyribonukleotidy chemie MeSH
- radioisotopová diluční technika MeSH
- radioizotopy fosforu * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 1-phenylazo-2-naphthol MeSH Prohlížeč
- DNA MeSH
- endonukleasy specifické pro jednořetězcové nukleové kyseliny MeSH
- karcinogeny MeSH
- naftoly MeSH
- polydeoxyribonukleotidy MeSH
- radioizotopy fosforu * MeSH
A 32P-postlabeling assay was employed for detection and quantitation of DNA adducts formed with carcinogenic 1-phenylazo-2-hydroxynaphthalene (Sudan I, Solvent Yellow 14) activated by a peroxidase system. Enrichment of adducts by digestion with nuclease P1 or by extraction into n-butanol prior to 32P-labeling was used. Both enrichment procedures exhibited comparable results for recovery of individual DNA adduct spots. Co-chromatographic analyses of adduct spots obtained by reaction with DNA and homopolydeoxy-ribonucleotides showed that four out of the eight major Sudan I-DNA adducts were formed by reaction of activated Sudan I with deoxyadenosine or deoxyguanosine in DNA. The accuracy of quantitation of adducts by 32P-postlabeling procedure is discussed.
Citace poskytuje Crossref.org
