The dihydropyridine receptor: expression of 190 kD alpha 1 subunit in crayfish muscle
Language English Country Slovakia Media print
Document type Journal Article
PubMed
1655559
Knihovny.cz E-resources
- MeSH
- Calcium Channel Blockers metabolism MeSH
- Gene Expression MeSH
- Protein Conformation MeSH
- RNA, Messenger genetics MeSH
- Molecular Weight MeSH
- Receptors, Nicotinic chemistry genetics metabolism MeSH
- Astacoidea metabolism MeSH
- Muscles metabolism MeSH
- Calcium Channels MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Calcium Channel Blockers MeSH
- RNA, Messenger MeSH
- Receptors, Nicotinic MeSH
- Calcium Channels MeSH
The existence of dihydropyridine receptor in crayfish striated muscle was proved by Northern blot analysis and 3H PN 200--110 binding. The alpha 1 subunit is encoded by a 8300 nt mRNA population and is expressed as 190 kD protein in crayfish T-tubular system, which binds 3H PN 200--110 (Bmax 1.5 +/- 0.4 pmol/mg protein and KD 6.2 +/- 0.8 nmol/l). The purified protein is phosphorylated by cAMP-dependent protein kinase. The dihydropyridine receptor in crayfish striated muscle also contains alpha 2 subunit, which on Northern blot gives the same signal as the alpha 2 subunit from rabbit skeletal muscle.
