Monoclonal antibodies to tick-borne encephalitis (TBE) virus: their use for differentiation of the TBE complex viruses
Language English Country Switzerland Media print
Document type Comparative Study, Journal Article
PubMed
1686961
Knihovny.cz E-resources
- MeSH
- Antigens, Viral immunology MeSH
- Antibodies, Monoclonal immunology MeSH
- Antibodies, Viral immunology MeSH
- Viral Proteins immunology MeSH
- Virulence MeSH
- Encephalitis Viruses, Tick-Borne classification immunology pathogenicity MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- Antigens, Viral MeSH
- Antibodies, Monoclonal MeSH
- Antibodies, Viral MeSH
- Viral Proteins MeSH
Monoclonal antibodies (MoAbs) to Central European tick-borne encephalitis virus (strain Hypr) were used for differentiation of eight viruses of the TBE complex by indirect immunofluorescence. MoAb 11/B3 (in Western blot recognizing 52 and 70 kD polypeptides) reacted with five out of the eight TBE complex viruses, MoAb 13/E5 (anti-52 kD protein) reacted with the western or eastern subtype of TBE virus only, while MoAb 12/G4 (anti-70 kD protein) distinguished the western subtype of TBE virus from the rest of the TBE complex. These three MoAbs were able to differentiate the virulent strain Hypr from attenuated strains Skalica and Hy-HK-18-"3". MoAb 2/10C (anti-56 and 70 kD proteins) which reacted with all viruses of the TBE complex, recognized both virulent and attenuated strains of TBE virus.
History of Arbovirus Research in the Czech Republic
