Monoclonal antibodies to tick-borne encephalitis (TBE) virus: their use for differentiation of the TBE complex viruses
Jazyk angličtina Země Švýcarsko Médium print
Typ dokumentu srovnávací studie, časopisecké články
PubMed
1686961
Knihovny.cz E-zdroje
- MeSH
- antigeny virové imunologie MeSH
- monoklonální protilátky imunologie MeSH
- protilátky virové imunologie MeSH
- virové proteiny imunologie MeSH
- virulence MeSH
- viry klíšťové encefalitidy klasifikace imunologie patogenita MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- antigeny virové MeSH
- monoklonální protilátky MeSH
- protilátky virové MeSH
- virové proteiny MeSH
Monoclonal antibodies (MoAbs) to Central European tick-borne encephalitis virus (strain Hypr) were used for differentiation of eight viruses of the TBE complex by indirect immunofluorescence. MoAb 11/B3 (in Western blot recognizing 52 and 70 kD polypeptides) reacted with five out of the eight TBE complex viruses, MoAb 13/E5 (anti-52 kD protein) reacted with the western or eastern subtype of TBE virus only, while MoAb 12/G4 (anti-70 kD protein) distinguished the western subtype of TBE virus from the rest of the TBE complex. These three MoAbs were able to differentiate the virulent strain Hypr from attenuated strains Skalica and Hy-HK-18-"3". MoAb 2/10C (anti-56 and 70 kD proteins) which reacted with all viruses of the TBE complex, recognized both virulent and attenuated strains of TBE virus.
History of Arbovirus Research in the Czech Republic
