Evaluation of restriction endonuclease cleavage of plant nuclear DNA using contaminating chloroplast DNA
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
1688020
Knihovny.cz E-resources
- MeSH
- Chloroplasts * MeSH
- DNA Probes MeSH
- Plants, Toxic MeSH
- Polymorphism, Restriction Fragment Length * MeSH
- DNA, Recombinant genetics MeSH
- Plants genetics MeSH
- Nicotiana MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA Probes MeSH
- DNA, Recombinant MeSH
Plant DNA is often contaminated by chloroplast DNA. The contamination was used here with advantage to check restriction endonuclease cleavage of plant DNA. This method is based on hybridization of Southern blots of digested plant DNA with cloned fragments of chloroplast DNA as a probe. Advantages of this method are demonstrated.
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