Quantification of proteins in sample buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using colloidal silver
Electrophoresis.
1991 Jun ;
12 (6) :
453-6.
Language English Country Germany Media print
Document type Journal Article
Persistent link
https://www.medvik.cz/link/pmid01716200
- MeSH
- Staining and Labeling * methods MeSH
- Densitometry MeSH
- Sodium Dodecyl Sulfate * MeSH
- Silver Nitrate * MeSH
- Electrophoresis, Polyacrylamide Gel * methods MeSH
- Collodion MeSH
- Colloids MeSH
- Membranes, Artificial MeSH
- Proteins chemistry MeSH
- Buffers MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Sodium Dodecyl Sulfate * MeSH
- Silver Nitrate * MeSH
- Collodion MeSH
- Colloids MeSH
- Membranes, Artificial MeSH
- Proteins MeSH
- Buffers MeSH
A rapid and simple assay for quantification of proteins in sodium dodecyl sulfate-sample buffer is described. Proteins are bound to a nitrocellulose membrane, stained with colloidal silver, and quantified by transmission densitometry. The staining requires 1 microL of protein sample and takes less than 20 min. Good linearity between the staining intensity and amount of proteins is in the range of 5-100 ng.
References provided by Crossref.org
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[epub] 20250402
Frontiers in immunology.
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1321321.
[epub] 20240202
γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis
Histochemistry.
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495-502.
