Resistance of Streptomyces cinnamonensis to butyrate and isobutyrate: production and properties of a new anti-isobutyrate (AIB) factor
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články
- MeSH
- antibiotická rezistence genetika MeSH
- biologické faktory analýza metabolismus MeSH
- butyráty antagonisté a inhibitory farmakologie MeSH
- isobutyráty MeSH
- kyselina máselná MeSH
- monensin biosyntéza MeSH
- mutace MeSH
- Streptomyces účinky léků genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- biologické faktory MeSH
- butyráty MeSH
- isobutyráty MeSH
- isobutyric acid MeSH Prohlížeč
- kyselina máselná MeSH
- monensin MeSH
Butyrate and isobutyrate (after isomerization to n-butyrate) are specific precursors for the biosynthesis of monensin A in Streptomyces cinnamonensis. High concentrations of both butyrate and isobutyrate (greater than 20 and 10 mM, respectively) were toxic to S. cinnamonensis plated on solid medium. Spontaneous mutants resistant to these substances were isolated. These new strains produced monensins at even higher concentrations of butyrate or isobutyrate, with an increased yield of monensin A. S. cinnamonensis produced an anti-isobutyrate (AIB) factor, which was originally found to be excreted by some isobutyrate-resistant stains growing on solid medium containing isobutyrate. On plates, the AIB factor efficiently counteracted toxic concentrations not only of isobutyrate, but also of acetate, propionate, butyrate, 2-methylbutyrate, valerate and isovalerate against S. cinnamonensis as well as other Streptomyces species. Although the AIB factor enabled normal growth, sporulation and monensin production on plates, it did not have positive effects on submerged cultures of S. cinnamonensis with isobutyrate. The partial purification of the AIB factor was achieved. The role of the AIB factor during spore germination on solid medium containing isobutyrate or its homologues is discussed.
Citace poskytuje Crossref.org
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