Produkcia kyseliny mliecnej a ureázová aktivita u bachorových kmenov Enterococcus faecium a ich genetická stabilita
[Lactic acid production and urease activity in strains of Enterococcus faecium found in the rumen and their genetic stability]
Jazyk slovenština Země Česko Médium print
Typ dokumentu anglický abstrakt, časopisecké články
PubMed
1807029
- MeSH
- bachor mikrobiologie MeSH
- Enterococcus faecium genetika izolace a purifikace metabolismus MeSH
- kyselina mléčná MeSH
- laktáty biosyntéza MeSH
- ovce mikrobiologie MeSH
- skot mikrobiologie MeSH
- ureasa metabolismus MeSH
- zvířata MeSH
- Check Tag
- skot mikrobiologie MeSH
- zvířata MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- kyselina mléčná MeSH
- laktáty MeSH
- ureasa MeSH
Lactic acid production, urease activity and genetic stability were investigated in five selected rumen strains of Enterococcus faecium. The average value of urease activity in the tested strains was 16.5 +/- 0.953 nkat per ml, two strains were urease-negative. The values of E. faecium strains produced lactic acid ranged from 1.087 +/- 0.134 to 1.787 +/- 0.213 mmol per 1 l. Cultivation of the strains in ethidium bromide (EB) eliminated urease activity of these strains already in the first subculture, but the elimination effects of sodium dodecyl sulphate (SDS) and acridine orange (AO) were manifested later on (1-8 subcultures). Lactic acid production was eliminated in all strains from 1st to 8th subculture after cultivation in SDS, EB and also AO.
