Production of laccase by Curvularia sp
Language English Country United States Media print
Document type Journal Article
PubMed
1822835
DOI
10.1007/bf02814506
Knihovny.cz E-resources
- MeSH
- Hydrogen-Ion Concentration MeSH
- Laccase MeSH
- Mitosporic Fungi enzymology growth & development MeSH
- Oxidoreductases biosynthesis MeSH
- Temperature MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Laccase MeSH
- Oxidoreductases MeSH
A Curvularia sp. isolated from soil was found to contain laccase activity toward guaiacol as substrate. The organism produced an extracellular laccase in a medium containing yeast extract, peptone and dextrose. Initial medium pH 4.0 and cultivation temperature 30 degrees C were found to be most suitable for maximum enzyme production. The optimum pH and temperature for laccase activity were found to be 5.2 and 50 degrees C, respectively. Under optimum conditions, the enzyme had a Km (guaiacol) of 0.75 mmol/L and a V of 1.50 CU min-1 ml-1. Some divalent metal ions inhibited laccase activity at very low concentrations.
Production of laccase enzyme from Curvularia lunata MY3: purification and characterization
Immobilized beta-glucosidase from Curvularia lunata
