Production of laccase by Curvularia sp
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
1822835
DOI
10.1007/bf02814506
Knihovny.cz E-zdroje
- MeSH
- koncentrace vodíkových iontů MeSH
- lakasa MeSH
- mitosporické houby enzymologie růst a vývoj MeSH
- oxidoreduktasy biosyntéza MeSH
- teplota MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- lakasa MeSH
- oxidoreduktasy MeSH
A Curvularia sp. isolated from soil was found to contain laccase activity toward guaiacol as substrate. The organism produced an extracellular laccase in a medium containing yeast extract, peptone and dextrose. Initial medium pH 4.0 and cultivation temperature 30 degrees C were found to be most suitable for maximum enzyme production. The optimum pH and temperature for laccase activity were found to be 5.2 and 50 degrees C, respectively. Under optimum conditions, the enzyme had a Km (guaiacol) of 0.75 mmol/L and a V of 1.50 CU min-1 ml-1. Some divalent metal ions inhibited laccase activity at very low concentrations.
Production of laccase enzyme from Curvularia lunata MY3: purification and characterization
Immobilized beta-glucosidase from Curvularia lunata
