Determination of nitrate by conversion to nitrite using Paracoccus denitrificans
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
1823647
DOI
10.1007/bf02814491
Knihovny.cz E-zdroje
- MeSH
- dusičnany analýza metabolismus MeSH
- dusitany metabolismus MeSH
- mutageneze MeSH
- nitrátreduktasa MeSH
- nitrátreduktasy metabolismus MeSH
- nitritreduktasy genetika metabolismus MeSH
- Paracoccus denitrificans genetika metabolismus MeSH
- stabilita enzymů MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- dusičnany MeSH
- dusitany MeSH
- nitrátreduktasa MeSH
- nitrátreduktasy MeSH
- nitritreduktasy MeSH
A new method of determination of nitrate was developed, utilizing the nitrate reductase activity of Paracoccus denitrificans in which a further reduction of nitrate is blocked either by a mutation affecting formation of cytochromes c or by inhibition of the electron flow to nitrite reductase by mucidin. After deproteinization of the sample with zinc acetate the nitrite produced is determined colorimetrically.
