Determination of nitrate by conversion to nitrite using Paracoccus denitrificans
Language English Country United States Media print
Document type Journal Article
PubMed
1823647
DOI
10.1007/bf02814491
Knihovny.cz E-resources
- MeSH
- Nitrates analysis metabolism MeSH
- Nitrites metabolism MeSH
- Mutagenesis MeSH
- Nitrate Reductase MeSH
- Nitrate Reductases metabolism MeSH
- Nitrite Reductases genetics metabolism MeSH
- Paracoccus denitrificans genetics metabolism MeSH
- Enzyme Stability MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Nitrates MeSH
- Nitrites MeSH
- Nitrate Reductase MeSH
- Nitrate Reductases MeSH
- Nitrite Reductases MeSH
A new method of determination of nitrate was developed, utilizing the nitrate reductase activity of Paracoccus denitrificans in which a further reduction of nitrate is blocked either by a mutation affecting formation of cytochromes c or by inhibition of the electron flow to nitrite reductase by mucidin. After deproteinization of the sample with zinc acetate the nitrite produced is determined colorimetrically.
