The use of some fluorescent stains for studying morphogenesis of micromycetes
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
1841854
DOI
10.1007/bf02935829
Knihovny.cz E-zdroje
- MeSH
- fluorescenční barviva * toxicita MeSH
- mykologie metody MeSH
- Saccharomyces cerevisiae účinky léků růst a vývoj MeSH
- Trichoderma účinky léků růst a vývoj MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fluorescenční barviva * MeSH
The use of some classical fluorochromes and optical brighteners in the fluorescence microscopy of micromycetes was investigated. Of the 16 compounds tested on slide cultures of Trichoderma viride 3 were too toxic, whereas the other stained primarily hyphae with various intensity. Reproductive structures did not stain or stained only weakly. With respect to vital staining the optical brightener Blankophor RKH exhibited most favorable properties. It did not inhibit either the growth or sporulation and stained intensively hyphae, septa and growth apices in particular. It also induced intensive fluorescence of a growing yeast culture.
Zobrazit více v PubMed
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