The effect of cyclophosphamide and gamma irradiation on adenosine deaminase and purine nucleoside phosphorylase in mice
Language English Country Netherlands Media print
Document type Journal Article
PubMed
1943447
DOI
10.1016/0024-3205(91)90392-o
PII: 0024-3205(91)90392-O
Knihovny.cz E-resources
- MeSH
- Adenosine Deaminase metabolism radiation effects MeSH
- Whole-Body Irradiation MeSH
- Cyclophosphamide pharmacology MeSH
- Injections, Intraperitoneal MeSH
- Mice, Inbred C57BL MeSH
- Mice, Inbred CBA MeSH
- Mice MeSH
- Purine-Nucleoside Phosphorylase metabolism radiation effects MeSH
- Spleen drug effects enzymology radiation effects MeSH
- Thymus Gland drug effects enzymology radiation effects MeSH
- Organ Size MeSH
- Chromatography, High Pressure Liquid MeSH
- Gamma Rays MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Adenosine Deaminase MeSH
- Cyclophosphamide MeSH
- Purine-Nucleoside Phosphorylase MeSH
Changes in ADA and PNP activities in the spleens and thymuses of mice were studied after a single administration of cyclophosphamide (CY, 200 mg/kg) and after whole-body gamma irradiation (5.5 Gy), applied alone or three days after CY application. In the first days after the treatment the enzyme activities were significantly depressed (p less than 0.01) with the exception of ADA in the spleen, where a high elevation (220-380%) in relation to controls was observed. During the regeneration period a pronounced rise of PNP activity in the spleen occurred mainly after a combined application of CY and irradiation (270%). In the thymus the regeneration was manifested by a mild increase of both ADA and PNP activities towards control values. The findings suggest that the expressive changes of ADA and PNP activities, participating in the purine salvage pathway, may, after a cytotoxic treatment, influence the nucleotide pool and DNA synthesis in lymphoid organs.
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