Detection of single-strand breaks in DNA induced by mitoxantrone in experimental tumors after in vivo treatment
Jazyk angličtina Země Slovensko Médium print
Typ dokumentu srovnávací studie, časopisecké články
PubMed
2011205
Knihovny.cz E-zdroje
- MeSH
- časové faktory MeSH
- experimentální nádory farmakoterapie genetika MeSH
- injekce intraperitoneální MeSH
- játra účinky léků MeSH
- jednovláknová DNA účinky léků MeSH
- ledviny účinky léků MeSH
- mitoxantron aplikace a dávkování terapeutické užití MeSH
- myši MeSH
- poškození DNA * MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- jednovláknová DNA MeSH
- mitoxantron MeSH
The fluorometric assay of DNA alkaline unwinding [15] was applied to murine tumor DNA after in vivo treatment. Mitoxantrone (MX) whose cytostatic effect has been extensively investigated was tested as DNA damaging compound. The single-strand breaks of DNA (SSB) in ascitic tumors (EAT. P388) and in solid tumors (B16a, B16) were detected after intraperitoneal administrations of 1 and 10 mg/kg MX in the course of 1 to 96 hours. The maximal number of SSB was measured after 1 to 6 hours and DNA damage outlasted 24 to 96 hours in dependence on the dose of MX and on the type of tumor. A dependence of DNA damage induced by MX on the route of tumor implantation was found. Ascitic tumor P388 and EAT were more than 5 times more sensitive to the effects of MX than solid melanomas. The SSB correlated well with the intracellular concentration of MX in the EAT cells.
