Detection of single-strand breaks in DNA induced by mitoxantrone in experimental tumors after in vivo treatment
Language English Country Slovakia Media print
Document type Comparative Study, Journal Article
PubMed
2011205
Knihovny.cz E-resources
- MeSH
- Time Factors MeSH
- Neoplasms, Experimental drug therapy genetics MeSH
- Injections, Intraperitoneal MeSH
- Liver drug effects MeSH
- DNA, Single-Stranded drug effects MeSH
- Kidney drug effects MeSH
- Mitoxantrone administration & dosage therapeutic use MeSH
- Mice MeSH
- DNA Damage * MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- DNA, Single-Stranded MeSH
- Mitoxantrone MeSH
The fluorometric assay of DNA alkaline unwinding [15] was applied to murine tumor DNA after in vivo treatment. Mitoxantrone (MX) whose cytostatic effect has been extensively investigated was tested as DNA damaging compound. The single-strand breaks of DNA (SSB) in ascitic tumors (EAT. P388) and in solid tumors (B16a, B16) were detected after intraperitoneal administrations of 1 and 10 mg/kg MX in the course of 1 to 96 hours. The maximal number of SSB was measured after 1 to 6 hours and DNA damage outlasted 24 to 96 hours in dependence on the dose of MX and on the type of tumor. A dependence of DNA damage induced by MX on the route of tumor implantation was found. Ascitic tumor P388 and EAT were more than 5 times more sensitive to the effects of MX than solid melanomas. The SSB correlated well with the intracellular concentration of MX in the EAT cells.
