An efficient method for isolation of plasmid DNA from methylotrophic bacteria
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
2125292
DOI
10.1007/bf02821415
Knihovny.cz E-zdroje
- MeSH
- DNA bakterií izolace a purifikace MeSH
- kruhová DNA izolace a purifikace MeSH
- methanol metabolismus MeSH
- Methylococcaceae genetika metabolismus MeSH
- oxidace-redukce MeSH
- plazmidy * MeSH
- půdní mikrobiologie * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- DNA bakterií MeSH
- kruhová DNA MeSH
- methanol MeSH
A method, suitable for the isolation of closed circular plasmid DNA from methylotrophic bacteria is described. Improvement of cell lysis was achieved by butanol extraction of cells before application of the lytic agent. Using this method, cryptic plasmids of 7.8, 14, 36 and 200 kb were purified from soil-isolated methylotrophs.
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Characterization of new plasmids from methylotrophic bacteria
