An efficient method for isolation of plasmid DNA from methylotrophic bacteria
Language English Country United States Media print
Document type Journal Article
PubMed
2125292
DOI
10.1007/bf02821415
Knihovny.cz E-resources
- MeSH
- DNA, Bacterial isolation & purification MeSH
- DNA, Circular isolation & purification MeSH
- Methanol metabolism MeSH
- Methylococcaceae genetics metabolism MeSH
- Oxidation-Reduction MeSH
- Plasmids * MeSH
- Soil Microbiology * MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA, Bacterial MeSH
- DNA, Circular MeSH
- Methanol MeSH
A method, suitable for the isolation of closed circular plasmid DNA from methylotrophic bacteria is described. Improvement of cell lysis was achieved by butanol extraction of cells before application of the lytic agent. Using this method, cryptic plasmids of 7.8, 14, 36 and 200 kb were purified from soil-isolated methylotrophs.
See more in PubMed
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Intergeneric conjugal transfer of Escherichia coli/Methylobacterium sp. shuttle vector
Characterization of new plasmids from methylotrophic bacteria
