Cystic fibrosis marker testing in Bohemia with polymerase chain reaction
Acta Universitatis Carolinae. Medica.
1990 ;
36 (1-4) :
108-11.
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
Persistent link
https://www.medvik.cz/link/pmid02130670
PubMed
2130670
Knihovny.cz E-resources
- MeSH
- Alleles MeSH
- Cystic Fibrosis diagnosis genetics MeSH
- DNA genetics MeSH
- Gene Frequency MeSH
- Genetic Markers MeSH
- Humans MeSH
- Polymerase Chain Reaction MeSH
- Prenatal Diagnosis MeSH
- Pregnancy MeSH
- Check Tag
- Humans MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA MeSH
- Genetic Markers MeSH
Conditions for assessing KM-19 probe detected by Pst-1 restriction fragment length polymorphism (RFLP) by means of polymerase chain reaction were provided. Computer controlled mechanical arm with waterbaths and cloned heat-stable DNA polymerase was used. Results of KM-19 allelic frequencies on 90 cystic fibrosis chromosomes are presented. Allele two frequency was -0.833.
