Construction of a promoter-probe shuttle vector for Escherichia coli and brevibacteria
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články
PubMed
2253886
DOI
10.1016/0378-1119(90)90424-p
PII: 0378-1119(90)90424-P
Knihovny.cz E-zdroje
- MeSH
- Brevibacterium genetika MeSH
- Escherichia coli genetika MeSH
- genetické vektory * MeSH
- léková rezistence genetika MeSH
- molekulární sekvence - údaje MeSH
- plazmidy * MeSH
- promotorové oblasti (genetika) genetika MeSH
- restrikční mapování MeSH
- sekvence nukleotidů MeSH
- transformace genetická MeSH
- Publikační typ
- časopisecké články MeSH
We constructed a promoter-probe vector, pJUP05, for brevibacteria and Escherichia coli based on the promoterless neomycin-resistance (neoR) gene from Tn5. This gene confers resistance to the aminoglycosides, kanamycin and neomycin. The promoter of the neoR gene was deleted and replaced by a suitable multiple cloning site. There are translation stop codons in all three reading frames upstream from the neoR gene. The plasmid contains functional origins of DNA replication for both brevibacteria and E. coli, and permits selection for chloramphenicol- and/or ampicillin-resistance markers.
Citace poskytuje Crossref.org
Construction and characterization of new corynebacterial plasmids carrying the alpha-amylase gene
