Construction of promoter-probe shuttle vectors for Escherichia coli and corynebacteria on the basis of promoterless alpha-amylase gene
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
11271817
DOI
10.1007/bf02817408
Knihovny.cz E-zdroje
- MeSH
- alfa-amylasy genetika metabolismus MeSH
- Bacillus subtilis enzymologie genetika MeSH
- bakteriální transformace * MeSH
- Corynebacterium genetika MeSH
- DNA sondy MeSH
- Escherichia coli genetika MeSH
- genetické vektory * MeSH
- plazmidy genetika MeSH
- promotorové oblasti (genetika) MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- alfa-amylasy MeSH
- DNA sondy MeSH
We constructed new promoter-probe vectors for E. coli and corynebacteria based on the promoterless alpha-amylase gene originating from Bacillus subtilis. Vectors pJUPAE1 and pJUPAE2 are suitable for isolation of transcriptionally active fragments from plasmids, phages or genomic DNA. alpha-Amylase activity can be easily visually detected on agar plates containing a chromogenic substrate, or by direct measurement of alpha-amylase activity.
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