Construction of promoter-probe shuttle vectors for Escherichia coli and corynebacteria on the basis of promoterless alpha-amylase gene
Language English Country United States Media print
Document type Journal Article
PubMed
11271817
DOI
10.1007/bf02817408
Knihovny.cz E-resources
- MeSH
- alpha-Amylases genetics metabolism MeSH
- Bacillus subtilis enzymology genetics MeSH
- Transformation, Bacterial * MeSH
- Corynebacterium genetics MeSH
- DNA Probes MeSH
- Escherichia coli genetics MeSH
- Genetic Vectors * MeSH
- Plasmids genetics MeSH
- Promoter Regions, Genetic MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- alpha-Amylases MeSH
- DNA Probes MeSH
We constructed new promoter-probe vectors for E. coli and corynebacteria based on the promoterless alpha-amylase gene originating from Bacillus subtilis. Vectors pJUPAE1 and pJUPAE2 are suitable for isolation of transcriptionally active fragments from plasmids, phages or genomic DNA. alpha-Amylase activity can be easily visually detected on agar plates containing a chromogenic substrate, or by direct measurement of alpha-amylase activity.
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