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Production and characteristics of the exocellular polysaccharide of a mutant Mycobacterium strain

. 1990 ; 35 (5) : 394-401.

Language English Country United States Media print

Document type Journal Article

Mutant strains of Mycobacterium sp. V-649 producing highly mucous colonies on a solid cultivation medium were prepared after treatment with N-methyl-N'-nitro-N-nitrosoguanidine and production of the exocellular polysaccharide was tested. The strains were cultivated in media with suitable sugar sources under submerged conditions. It was found that Mycobacterium sp. V-649/15 produces a maximum of 15-19% polymer after a 5-6-d cultivation. Gas chromatography indicated that the exocellular polysaccharide produced by this strain is of glucan type.

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