Monoclonal antibodies against hepatitis B e antigen: production, characterization, and use for diagnosis
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články
PubMed
2370287
DOI
10.1016/0166-0934(90)90031-a
PII: 0166-0934(90)90031-A
Knihovny.cz E-zdroje
- MeSH
- buněčné linie MeSH
- hepatitida B - antigeny e imunologie MeSH
- hepatitida B - antigeny povrchové imunologie MeSH
- hepatitida B - protilátky analýza MeSH
- hepatitida B diagnóza MeSH
- hybridomy imunologie MeSH
- lidé MeSH
- monoklonální protilátky imunologie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- rekombinantní proteiny imunologie MeSH
- virus hepatitidy B imunologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- hepatitida B - antigeny e MeSH
- hepatitida B - antigeny povrchové MeSH
- hepatitida B - protilátky MeSH
- monoklonální protilátky MeSH
- rekombinantní proteiny MeSH
Five different hybridoma clones secreting anti-HBeAg antibody were constructed by fusing cells of mouse myeloma line SP2/0 with splenocytes from BALB/c mice immunized with recombinant HBeAg. The monoclonal antibodies obtained were characterized immunologically and one was used to develop ELISA for detection of HBeAg and anti-HBeAg antibody. These monoclonal assays enabled the detection of 3 U HBeAg/ml and 1 U anti-HBeAg/ml with reference to standards of the Paul Ehrlich Institute, Frankfurt, F.R.G. Both assays compared well with a commercially available kit (Abbott Laboratory) and were used for detection of HBeAg and anti-HBeAg antibody in clinical serum samples.
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