Lack of synthesis of pentamer IgM in Xenopus oocytes after injection of poly(A)+ RNA from hybridoma cells
Folia biologica.
1987 ;
33 (2) :
81-6.
Jazyk angličtina Země Česko Médium print
Typ dokumentu srovnávací studie, časopisecké články
Perzistentní odkaz
https://www.medvik.cz/link/pmid02439387
PubMed
2439387
Knihovny.cz E-zdroje
- MeSH
- B-lymfocyty imunologie MeSH
- hybridomy imunologie MeSH
- imunoglobulin G biosyntéza MeSH
- imunoglobulin M biosyntéza genetika MeSH
- messenger RNA genetika MeSH
- oocyty metabolismus MeSH
- ovce MeSH
- poly A genetika MeSH
- proteosyntéza MeSH
- RNA genetika MeSH
- Xenopus MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- imunoglobulin G MeSH
- imunoglobulin M MeSH
- messenger RNA MeSH
- poly A MeSH
- RNA MeSH
Total poly(A)+ RNA was isolated from B cell hybridomas producing monoclonal antibodies of the IgM class specific for sheep red blood cells. These RNAs were microinjected into Xenopus oocytes and the translation products were analyzed. Unlike the hybridoma cells, the oocytes synthetized only the light chains and oligomer IgM, but evidence for assembly of functional pentamer IgM was not obtained. The possible reasons for these differences are discussed.
