Regulation of extracellular proteins and alpha-amylase secretion by temperature in Bacillus subtilis
Language English Country United States Media print
Document type Journal Article
PubMed
2509311
DOI
10.1007/bf02821290
Knihovny.cz E-resources
- MeSH
- alpha-Amylases biosynthesis metabolism MeSH
- Bacillus subtilis enzymology metabolism MeSH
- Bacterial Proteins biosynthesis MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Leucine metabolism MeSH
- Temperature MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- alpha-Amylases MeSH
- Bacterial Proteins MeSH
- Leucine MeSH
alpha-Amylase was found to be the main protein secreted by Bacillus subtilis, corresponding to 90, 87 and 60% of total extracellular proteins at 30, 40 and 45 degrees C, respectively. A change in temperature can affect the pattern of proteins secreted as detected by gel electrophoresis. 14C-Leucine incorporation into extracellular proteins and their proportion at the end of the growth phase was higher at 30 degrees C than that at 40 or 45 degrees C. The effect of temperature on alpha-amylase synthesis as determined by its enzymic activity and on the extracellular protein synthesis followed a similar pattern.
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