Concentration of poliovirus type I from cow milk using an adsorption-elution method
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
2553805
Knihovny.cz E-resources
- MeSH
- Adsorption MeSH
- Alum Compounds MeSH
- Virus Cultivation MeSH
- Milk microbiology MeSH
- Specimen Handling MeSH
- Poliovirus isolation & purification MeSH
- Vero Cells MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- aluminum sulfate MeSH Browser
- Alum Compounds MeSH
Cow milk was experimentally contaminated with a vaccine strain of poliovirus type I. A concentration procedure was utilized to assay for the virus in a milk sample using adsorption on aluminium sulphate at pH 4.5-5.5, aluminium sulphate concentration being 0.15 g. 1(-1), followed by elution with 0.1 M Na2HPO4 pH 9.5 and subsequent detection in cultured VERO cells (kidney cells from the monkey Cercopithecus aethiops). The yield of the virus ranged from 14 to 58% of the inoculated amount, the mean value being 27%.
