A kinetic fluorometric assay of dipeptidyl peptidase IV in viable human blood mononuclear cells
Jazyk angličtina Země Francie Médium print
Typ dokumentu časopisecké články
PubMed
2568856
DOI
10.1016/0300-9084(89)90092-8
PII: 0300-9084(89)90092-8
Knihovny.cz E-zdroje
- MeSH
- dipeptidylpeptidasa 4 MeSH
- dipeptidylpeptidasy a tripeptidylpeptidasy krev MeSH
- DNA krev MeSH
- endopeptidasy krev MeSH
- fluorescenční spektrometrie MeSH
- kinetika MeSH
- leukocyty mononukleární enzymologie MeSH
- lidé MeSH
- prolyloligopeptidasy MeSH
- separace buněk MeSH
- serinové endopeptidasy * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- dipeptidylpeptidasa 4 MeSH
- dipeptidylpeptidasy a tripeptidylpeptidasy MeSH
- DNA MeSH
- endopeptidasy MeSH
- PREPL protein, human MeSH Prohlížeč
- prolyloligopeptidasy MeSH
- serinové endopeptidasy * MeSH
A continuous-rate fluorometric assay of dipeptidyl peptidase IV (DP-IV) in viable human blood mononuclear cells using 7-(L-glycyl-L-prolylamido)-4-methylcoumarin as the substrate is described. The assay method is accurate, rapid, and highly sensitive for measuring the level of cell-surface bound DP-IV activity in suspension of blood mononuclear cells, as well as of other viable cells bearing this enzyme. We believe that the kinetic assay is suitable for studying the regulation of expression and the role of plasma membrane-bound DP-IV on the cellular level.
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