Ultrastructural immunolocalization of cyclin/PCNA in synchronized 3T3 cells
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
2571512
DOI
10.1016/0014-4827(89)90366-2
PII: 0014-4827(89)90366-2
Knihovny.cz E-resources
- MeSH
- Cell Nucleus metabolism ultrastructure MeSH
- Cell Line MeSH
- Hydroxyurea pharmacology MeSH
- Immunohistochemistry MeSH
- Nuclear Proteins metabolism MeSH
- Cell Compartmentation MeSH
- Mice MeSH
- Proliferating Cell Nuclear Antigen MeSH
- DNA Replication * drug effects MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Hydroxyurea MeSH
- Nuclear Proteins MeSH
- Proliferating Cell Nuclear Antigen MeSH
The immunolocalization of cyclin/PCNA in synchronized 3T3 cells was performed with human autoantibodies using an immunogold technique performed on thin cryosections. Previous immunofluorescent studies demonstrated that the DNA replication sites correspond to the localization of bound cyclin. We have found that in the early periods of S phase, the DNA replication sites (or sites potentially ready for the replication during the hydroxyurea DNA synthesis block) are situated in the perichromatin region and correspond to clustered gold particles present frequently over a morphologically distinct small nuclear area. Heavily labeled chromocenters, including perinucleolar condensed chromatin, exhibiting several such distinct areas were found in later periods of S phase.
References provided by Crossref.org
Strengths and Weaknesses of Cell Synchronization Protocols Based on Inhibition of DNA Synthesis
DNA Replication: From Radioisotopes to Click Chemistry
