Ultrastructural immunolocalization of cyclin/PCNA in synchronized 3T3 cells
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
2571512
DOI
10.1016/0014-4827(89)90366-2
PII: 0014-4827(89)90366-2
Knihovny.cz E-zdroje
- MeSH
- buněčné jádro metabolismus ultrastruktura MeSH
- buněčné linie MeSH
- hydroxymočovina farmakologie MeSH
- imunohistochemie MeSH
- jaderné proteiny metabolismus MeSH
- kompartmentace buňky MeSH
- myši MeSH
- proliferační antigen buněčného jádra MeSH
- replikace DNA * účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- hydroxymočovina MeSH
- jaderné proteiny MeSH
- proliferační antigen buněčného jádra MeSH
The immunolocalization of cyclin/PCNA in synchronized 3T3 cells was performed with human autoantibodies using an immunogold technique performed on thin cryosections. Previous immunofluorescent studies demonstrated that the DNA replication sites correspond to the localization of bound cyclin. We have found that in the early periods of S phase, the DNA replication sites (or sites potentially ready for the replication during the hydroxyurea DNA synthesis block) are situated in the perichromatin region and correspond to clustered gold particles present frequently over a morphologically distinct small nuclear area. Heavily labeled chromocenters, including perinucleolar condensed chromatin, exhibiting several such distinct areas were found in later periods of S phase.
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