Unusual stage-specific embryonic antigen (TEC-4) defined by a monoclonal antibody to embryonal carcinoma cells defective in the expression of embryoglycan
Language English Country United States Media print
Document type Journal Article
PubMed
2574458
PubMed Central
PMC298490
DOI
10.1073/pnas.86.23.9337
Knihovny.cz E-resources
- MeSH
- Lewis X Antigen MeSH
- Antigens, Neoplasm isolation & purification MeSH
- Cell Differentiation drug effects MeSH
- Cell Line MeSH
- Cytotoxicity, Immunologic MeSH
- Gene Expression MeSH
- Fluorescent Antibody Technique MeSH
- Glycolipids genetics isolation & purification MeSH
- Molecular Weight MeSH
- Antibodies, Monoclonal * MeSH
- Mice MeSH
- Biomarkers, Tumor analysis MeSH
- Tumor Cells, Cultured cytology drug effects immunology MeSH
- Polysaccharides genetics MeSH
- Teratoma immunology MeSH
- Tretinoin pharmacology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Lewis X Antigen MeSH
- Antigens, Neoplasm MeSH
- embryoglycan MeSH Browser
- Glycolipids MeSH
- Antibodies, Monoclonal * MeSH
- Biomarkers, Tumor MeSH
- Polysaccharides MeSH
- Tretinoin MeSH
Most developmentally regulated epitopes identified on embryonal carcinoma cells and murine preimplantation embryos are associated with a glycoprotein-bound large glycan called embryoglycan. To prepare monoclonal antibodies recognizing other, less immunogenic stage-specific embryonic epitopes, we used embryoglycan-negative embryonal carcinoma cells P19XT.1.1 as immunogen. One monoclonal antibody prepared by this strategy was found to react specifically with mouse embryonal carcinoma and embryo-derived stem cell lines. The target epitope, TEC-4, was found to be expressed on eggs and two-cell embryos but was undetectable on later stages of mouse embryos and adult mouse tissues. NaDodSO4/PAGE of immunoaffinity-isolated antigen revealed that TEC-4 epitope is associated with glycoproteins of apparent Mr 120,000 and 240,000. The epitope was resistant to oxidation by sodium periodate and to digestion by endoglycosidase F but was sensitive to treatment with protein-denaturing agents and proteases, which suggested that the epitope is located in the protein moiety of the molecule. In the course of retinoic acid-induced differentiation of embryonal carcinoma cells the epitope disappeared before the onset of morphological differentiation. The combined data indicate that TEC-4 is an unusual stage-specific embryonic antigen that may be amenable to direct genetic analysis.
See more in PubMed
Dev Biol. 1982 Feb;89(2):503-8 PubMed
Science. 1988 May 13;240(4854):889-95 PubMed
Exp Cell Res. 1989 Feb;180(2):326-40 PubMed
Mol Immunol. 1989 Feb;26(2):153-61 PubMed
Int Arch Allergy Appl Immunol. 1966;29(2):185-9 PubMed
Nature. 1970 Aug 15;227(5259):680-5 PubMed
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3170-4 PubMed
Immunol Rev. 1977 Jan;33:3-32 PubMed
Nature. 1977 Apr 7;266(5602):550-2 PubMed
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5565-9 PubMed
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PubMed
Biochem J. 1980 Apr 1;187(1):1-20 PubMed
J Immunogenet. 1980 Dec;7(6):455-74 PubMed
J Immunol Methods. 1981;43(2):175-9 PubMed
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7634-8 PubMed
Int J Cancer. 1982 May 15;29(5):523-31 PubMed
J Cell Biol. 1982 Aug;94(2):253-62 PubMed
Cell. 1982 Oct;30(3):697-705 PubMed
Mol Cell Biol. 1983 Dec;3(12):2271-9 PubMed
Dev Biol. 1984 Jan;101(1):225-8 PubMed
Lab Invest. 1984 Feb;50(2):147-62 PubMed
J Biochem. 1985 Jan;97(1):307-15 PubMed
Cell Differ. 1984 Dec;15(2-4):109-13 PubMed
Cell Differ. 1985 May;16(3):169-73 PubMed
Differentiation. 1986;31(3):174-82 PubMed
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5798-802 PubMed
Cell Differ. 1987 Jul;21(2):119-30 PubMed
Immunogenetics. 1987;26(4-5):273-81 PubMed
J Cell Biochem. 1988 Jan;36(1):1-14 PubMed
Biochim Biophys Acta. 1988 Mar 11;968(3):291-9 PubMed
Differentiation. 1988 May;37(3):205-14 PubMed
