Unusual stage-specific embryonic antigen (TEC-4) defined by a monoclonal antibody to embryonal carcinoma cells defective in the expression of embryoglycan
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
2574458
PubMed Central
PMC298490
DOI
10.1073/pnas.86.23.9337
Knihovny.cz E-zdroje
- MeSH
- antigen Lewis X MeSH
- antigeny nádorové izolace a purifikace MeSH
- buněčná diferenciace účinky léků MeSH
- buněčné linie MeSH
- cytotoxicita imunologická MeSH
- exprese genu MeSH
- fluorescenční protilátková technika MeSH
- glykolipidy genetika izolace a purifikace MeSH
- molekulová hmotnost MeSH
- monoklonální protilátky * MeSH
- myši MeSH
- nádorové biomarkery analýza MeSH
- nádorové buňky kultivované cytologie účinky léků imunologie MeSH
- polysacharidy genetika MeSH
- teratom imunologie MeSH
- tretinoin farmakologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antigen Lewis X MeSH
- antigeny nádorové MeSH
- embryoglycan MeSH Prohlížeč
- glykolipidy MeSH
- monoklonální protilátky * MeSH
- nádorové biomarkery MeSH
- polysacharidy MeSH
- tretinoin MeSH
Most developmentally regulated epitopes identified on embryonal carcinoma cells and murine preimplantation embryos are associated with a glycoprotein-bound large glycan called embryoglycan. To prepare monoclonal antibodies recognizing other, less immunogenic stage-specific embryonic epitopes, we used embryoglycan-negative embryonal carcinoma cells P19XT.1.1 as immunogen. One monoclonal antibody prepared by this strategy was found to react specifically with mouse embryonal carcinoma and embryo-derived stem cell lines. The target epitope, TEC-4, was found to be expressed on eggs and two-cell embryos but was undetectable on later stages of mouse embryos and adult mouse tissues. NaDodSO4/PAGE of immunoaffinity-isolated antigen revealed that TEC-4 epitope is associated with glycoproteins of apparent Mr 120,000 and 240,000. The epitope was resistant to oxidation by sodium periodate and to digestion by endoglycosidase F but was sensitive to treatment with protein-denaturing agents and proteases, which suggested that the epitope is located in the protein moiety of the molecule. In the course of retinoic acid-induced differentiation of embryonal carcinoma cells the epitope disappeared before the onset of morphological differentiation. The combined data indicate that TEC-4 is an unusual stage-specific embryonic antigen that may be amenable to direct genetic analysis.
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Dev Biol. 1982 Feb;89(2):503-8 PubMed
Science. 1988 May 13;240(4854):889-95 PubMed
Exp Cell Res. 1989 Feb;180(2):326-40 PubMed
Mol Immunol. 1989 Feb;26(2):153-61 PubMed
Int Arch Allergy Appl Immunol. 1966;29(2):185-9 PubMed
Nature. 1970 Aug 15;227(5259):680-5 PubMed
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3170-4 PubMed
Immunol Rev. 1977 Jan;33:3-32 PubMed
Nature. 1977 Apr 7;266(5602):550-2 PubMed
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5565-9 PubMed
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PubMed
Biochem J. 1980 Apr 1;187(1):1-20 PubMed
J Immunogenet. 1980 Dec;7(6):455-74 PubMed
J Immunol Methods. 1981;43(2):175-9 PubMed
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7634-8 PubMed
Int J Cancer. 1982 May 15;29(5):523-31 PubMed
J Cell Biol. 1982 Aug;94(2):253-62 PubMed
Cell. 1982 Oct;30(3):697-705 PubMed
Mol Cell Biol. 1983 Dec;3(12):2271-9 PubMed
Dev Biol. 1984 Jan;101(1):225-8 PubMed
Lab Invest. 1984 Feb;50(2):147-62 PubMed
J Biochem. 1985 Jan;97(1):307-15 PubMed
Cell Differ. 1984 Dec;15(2-4):109-13 PubMed
Cell Differ. 1985 May;16(3):169-73 PubMed
Differentiation. 1986;31(3):174-82 PubMed
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5798-802 PubMed
Cell Differ. 1987 Jul;21(2):119-30 PubMed
Immunogenetics. 1987;26(4-5):273-81 PubMed
J Cell Biochem. 1988 Jan;36(1):1-14 PubMed
Biochim Biophys Acta. 1988 Mar 11;968(3):291-9 PubMed
Differentiation. 1988 May;37(3):205-14 PubMed
