Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol
Language English Country Great Britain, England Media print
Document type Journal Article
- MeSH
- Antigens, Surface immunology metabolism MeSH
- Cell Membrane immunology metabolism MeSH
- Cell Line MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Phosphatidylinositols metabolism MeSH
- Glycoproteins immunology metabolism MeSH
- Leukocytes immunology MeSH
- Humans MeSH
- Molecular Sequence Data MeSH
- Antibodies, Monoclonal immunology metabolism MeSH
- Mice MeSH
- Amino Acid Sequence MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Antigens, Surface MeSH
- Phosphatidylinositols MeSH
- Glycoproteins MeSH
- Antibodies, Monoclonal MeSH
A monoclonal antibody MEM-43 was prepared, which recognizes an antigen expressed on all peripheral blood leucocytes, on erythrocytes and several cell lines, but is absent from U937, Nalm-6, Daudi and Raji cell lines. The antigen isolated by immunoaffinity chromatography from several cell lines is an 18,000-25,000 mol. wt glycoprotein. An apparently identical antigen isolated from erythrocytes binds to several lectins and has a 14,000 mol. wt polypeptide backbone, modified by an endoglycosidase F-sensitive carbohydrate moiety. The epitope recognized is reduction-sensitive. The sequence of N-terminal 17 amino acid residues was determined; five out of six N-terminal amino acids are identical to those found at the N-terminus of the mouse lymphocyte surface antigen Ly-6C. The antigen is completely released from the cell surface after treatment with phosphatidylinositol-specific phospholipase C.
References provided by Crossref.org
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The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1
