Detection of antibodies against hepatitis B core antigen using the avidin-biotin system
Language English Country Netherlands Media print
Document type Comparative Study, Journal Article
PubMed
2668320
DOI
10.1016/0166-0934(89)90044-x
PII: 0166-0934(89)90044-X
Knihovny.cz E-resources
- MeSH
- Avidin * MeSH
- Biotin * MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Escherichia coli MeSH
- Hepatitis B Core Antigens genetics immunology MeSH
- Hepatitis B Antibodies analysis MeSH
- Hepatitis B diagnosis MeSH
- Cloning, Molecular MeSH
- Humans MeSH
- Radioimmunoassay MeSH
- Sensitivity and Specificity MeSH
- Hepatitis B virus immunology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- Avidin * MeSH
- Biotin * MeSH
- Hepatitis B Core Antigens MeSH
- Hepatitis B Antibodies MeSH
An enzyme avidin-biotin assay for the detection of anti-HBcAg antibody in human sera was developed. The assay uses genetically engineered HBcAg. HBcAg is immobilized on the surface of the wells of microtitre plates and the test serum sample, biotin-labelled HBcAg and streptavidin-labelled horseradish peroxidase are added. The assay was found to be specific and was compared with a commercial radioimmunoassay kit for sensitivity by testing 96 human clinical sera for anti-HBcAg antibody. Both assays gave identical results.
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