Characterization of phospholipase A2 of mycoplasma species
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
2691360
DOI
10.1007/bf02814471
Knihovny.cz E-zdroje
- MeSH
- Acholeplasma laidlawii enzymologie MeSH
- extracelulární prostor enzymologie MeSH
- fosfolipasy A izolace a purifikace metabolismus MeSH
- fosfolipasy A2 MeSH
- fosfolipasy metabolismus MeSH
- gelová chromatografie MeSH
- koncentrace vodíkových iontů MeSH
- Mycoplasma mycoides enzymologie MeSH
- vápník farmakologie MeSH
- vysoká teplota MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fosfolipasy A MeSH
- fosfolipasy A2 MeSH
- fosfolipasy MeSH
- vápník MeSH
As phospholipases of mycoplasma species may play a role in the pathogenesis of respiratory tract and urogenital tract diseases Mycoplasma mycoides and Acholeplasma laidlawii were examined as to the production of phospholipase A2 (PLA) and attempts were made to purify and characterize it. Both species produced PLA. The purified enzyme was found to be heat-labile, active at alkaline pH, revealing a single band in polyacrylamide gel electrophoresis. Metal ions such as calcium and barium, increased its activity whereas solvents at high concentrations decreased it. It was resistant to surfactants.
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